Year of grant:
2011

Research Area:
Náttúra og náttúrutilfeingi

Project type:
Ph.d.verkætlan

Project title:
Bioactive peptides - extraction and processing methods for marine secondary products

Grant number:
0428

Project manager:
Durita Dahl Djurhuus

Institution/company:
Atlantic Biotechnology og Náttúruvísindadeildin

Other participants:
Svein-Ole Mikalsen,Hóraldur Joensen og Jan Stagsted

Project period:
1.5.2011-30.4.2014

Total budget:
1.969.000 DKK

Grant from the FRC in DKK:
850.000 DKK

Project description:
Original
This Ph.D. project will focus on development of extraction and processing methods for bioactive peptides from marine secondary products. This includes both mild extraction procedures that preserve natural bioactive peptides as well as chemical/enzymatic hydrolysis to optimize production of various peptide fractions from high quality fish. Ultra- and nanofiltration of dissolved peptides will result in peptides with a nominal MW between 500 and 10.000 Dalton. These process methods are availiable at ABt (Atlantic Biotechnology). Ion exchange is planned to be included in the process in the future.

Different peptide fractions will be extracted on small scale and characterized using different chromatographic methods such as gel filtration or specific affinity chromatography and also LCMS and MS/MS. The peptides will be tested in biological test systems for their effect on e.g. antibacterial activity, wound healing and/or cancer cell proliferation and migration.

When positive results are obtained the process will be repeated on large scale and analysed using the same assays as for small scale trials. Large scale extraction of antimicrobial peptides will be used in combination with chitosan to test antimicrobial effect in the gastrointestinal tract of weaning piglets.

Project status:
Steðgað

Project output:
Due to different reasons the project was terminated before completion.



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